Coding

Part:BBa_K2210001:Design

Designed by: Bowen Xiao   Group: iGEM17_RDFZ-China   (2017-10-22)


lmrA-transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 697
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Molecular docking has been used to increase affinity to surfactin. For more information on the exact changes, see http://2017.igem.org/Team:RDFZ-China/Model

Source

L. lactis

References

Reuter, Galya, et al. "The ATP Binding Cassette Multidrug Transporter LmrA and Lipid Transporter MsbA Have Overlapping Substrate Specificities." Journal of Biological Chemistry 278.37 (2003): 35193-35198.